Accuris™ Taq High Fidelity HotStart DNA Polymerase, 25µl, PR1001-HFHS

Accuris™ High Fidelity HotStart PR1001-HFHS DNA Polymerase is a ready-to-use 2X PCR master mix designed for highly accurate and reliable DNA amplification, especially in applications where sequence fidelity is critical such as cloning, mutagenesis, and sequencing.

This hot-start formulation combines a genetically engineered proofreading DNA polymerase with a proprietary antibody-mediated hot-start system. The enzyme remains inactive at room temperature, helping prevent non-specific amplification and primer-dimer formation until the initial denaturation step, improving specificity and reproducibility.

The polymerase includes 3’→5’ exonuclease proofreading activity, enabling high-fidelity DNA synthesis with significantly reduced error rates compared to standard Taq polymerase. It also delivers strong performance on difficult templates, including GC-rich and low-copy-number DNA, and is optimized for efficient amplification of targets up to approximately 10 kb

Applications:

• Blunt End / TA Cloning
• Colony PCR
• Fast PCR
• GC- & AT-Rich DNA Amplification
• Low Copy PCR

Each mix includes:

• High Fidelity Polymerase
• Buffer [PCR Enhancers, 2mM dNTPs, 6mM MgCl2 , hot-start antibodies

Features:

• Hot-start activation for improved specificity and reduced non-specific products
• High fidelity DNA synthesis with proofreading activity
• Optimized 2X master mix format for faster setup and fewer pipetting steps
• Robust performance with complex, GC-rich, or low-quality templates
• Suitable for cloning, site-directed mutagenesis, sequencing, and general high-accuracy PCR

Accuris™ HFHS HotStart Taq DNA Protocol   Accuris DNA Polymerase Catalog